Background: Stroke is the second most common cause of death and may result in various disabilities; thus, identification\nof neuroprotective therapeutic agents is important. Peucedani Radix (PR), the root of Angelica decursiva, is\na well-known remedy for damp and phlegm in Korean medicine and has also been shown to exert antioxidant and\nanti-inflammatory activities. This study was performed to investigate the mechanism underlying the anti-inflammatory\neffect of methanol extract of PR (PRex) on cerebral ischemic injury.\nMethods: C57BL/6 male mice were orally administered PRex (20, 60, or 200 mg/kg) at 2 days, 1 day, and 1 h prior to\nmiddle cerebral artery occlusion (MCAO). Twenty-four hours after MCAO, the infarct volume was measured and the\nneurological deficit score was assessed. The inflammatory-related substances in the ipsilateral hemisphere were determined\nby western blotting, DCFH-DA assay, TBARS assay, and ELISA.\nResults: PRex pretreatment significantly decreased the infarct volume at 24 h after MCAO. Moreover, PRex effectively\nsuppressed the expression of iNOS, ROS, MDA, and pro-inflammatory cytokines, such as IL-1�² and TNF-�±, in brain tissue\nof mice with MCAO-induced brain injury.\nConclusions: PRex protected neurons from ischemic brain injury in mice through its antioxidant and anti-inflammatory\nactivities. Our results suggested that PR could be a promising candidate in the therapy of ischemia-induced brain\ndamage.
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